Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei

Cong⁃cong XING, Fei XU, Xin⁃li BAI, Jin⁃sheng FU, Wen⁃qian LI, Xiao⁃wei LU, Hai⁃yang MA, Zhen⁃zhong LI

Abstract


Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM⁃T⁃WaaF cloning plasmid. Downloaded five WaaF associated with Guillain⁃Barré syndrome (GBS) and one WaaF not associated with GBS, and then constructed phylogenetic tree. Results pGEM ⁃ T ⁃ WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobacter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang.

DOI：10.3969/j.issn.1672⁃6731.2012.02.013

Keywords


Campylobacter jejuni; Genes; Sequence analysis, DNA; Evolution; Genetics

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